The Holby City Hospital witnesses the ups and downs in the everyday lives of young doctors, nurses, and other staff as they attend to a multitude of patients in the A&E department.
Nurse Mads struggles on the first day of her new job when shootings at Holby College throw a fully stretched ED into turmoil.
The aftermath of the shootings and the dramatic incidents at Holby College leave everyone in the emergency department struggling to cope.
Jordan bears the brunt of an attack on the ED from the Press and public, while Ruth eagerly awaits news regarding her application for a surgical job.
Jordan rallies the team after introducing an unpopular new policy, and a boy is suspected of taking and selling LSD when he is admitted after falling from a school building.
Jordan declares the Clinical Decisions Unit open for business, but the new system leaves Adam struggling to keep up. Edward tries to apologise to Ruth.
As the staff prepare for the cleanliness inspection, Lenny makes several mistakes that jeopardise the ED's chances of success.
Jordan tries to improve the image of the department. Jeff is hailed as a hero, but cannot handle being the centre of attention. Simone's mother antagonises Kirsty.
On the day of the memorial service for those killed in the college attacks, Ruth struggles to manage the department, while Jeff and Simone both face their demons.
Ruth tries to prove she's the ideal candidate to become Jordan's deputy. Shona's disappearance forces Charlie to care for his grand-daughter, with dire consequences.
A mixture of drugs causes Kirsty to collapse, and after she has been secretly treated by Adam, her husband arrives to take her home.
Jordan takes drastic measures as the department is pushed to breaking point by a major incident and it becomes apparent it is likely to fail its inspection.
Charlie offers emotional support to Ruth after she discovers a devastating secret about her husband Edward, but the move comes at a huge cost to himself.
Ruth stands by her decision to move Charlie, but gets a frosty reception on her first day as clinical standards manager.
Jay starts a petition against an administrative adviser, while Ruth tries to persuade Edward they should come clean about their sham marriage.
Ruth undermines Zoe by revealing she's the doctor with the highest mortality rate in the ED. Lenny has a psychiatric evaluation before becoming his sister's donor.
Jeff retaliates against unscrupulous gang leader Karl Fontayne. Mads is torn between the values of her old and new lives. Lenny tries not to give his sister bad news.
Kirsty makes an important decision about her future after Warren is admitted to the ED. Yuki helps Lenny in his desperate search to find a donor match for his sister.
The precarious state of Ruth's marriage threatens to ruin her Christmas. Mads and Noel throw a party in the ED. Kirsty struggles to put aside her feelings for Adam.
Jordan gets behind the wheel of a car, with fatal consequences. New Year begins terribly for Ruth when she loses a patient and ruins a blossoming friendship.
A tense confrontation between Adam and Warren has brutal consequences for Kirsty, while Jordan is kidnapped at knife point and forced to treat the victim of a stabbing.
Ruth's fragmented memories of a drunken night point to the possibility she was raped, and she's horrified when the man she thinks attacked her is admitted to the ED.
Ruth's recent manic behaviour is exacerbated by the arrival of a patient with mental health problems, while Kirsty and Warren's wedding anniversary ends in disaster.
Kirsty prepares to leave Warren, and the staff are devastated they failed to notice the extent of Ruth's mental health problems.
Ruth is sectioned and held in a specialist unit, where consultant psychiatrist Andrew Brookfield tries to pinpoint the cause of her distress.
Jordan runs into problems while carrying out a surgical procedure on a Middle Eastern princess, much to the disapproval of Henrik Hanssen.
Warren wakes from his coma and confronts Adam about his affair with Kirsty, while Jordan clashes with the new joint clinical lead, who is his former mentor.
Nita tries to run away and joins a woman fleeing to Scarborough with her brother. Jay feels stressed after the cancer scare and receives an unwelcome visit from Maryam.
An interviewee for an ED job is involved in a traffic accident, Jay learns he has cancer and Ruth escapes from the psychiatric ward.
Jay tries to distract himself from his impending surgery, while Miriam and Jordan's constant bickering forces Hanssen to decide there should be only one clinical lead.
Ruth learns former mentor Sarah Evans has OCD, and realises she can still be a doctor despite her bipolar diagnosis.
Jay struggles to cope with work as he returns from his operation, and his grandmother's admittance to the ED only makes matters worse.
The competition between Jordan and Miriam intensifies as the board prepares to appoint one of them as sole clinical lead.
The truth about Warren's death is revealed when a distraught Nita scales a fire tower with a bottle of vodka.
Real-time episode that sees Polly's life hanging in the balance after being stabbed and left for dead in the basement by the unstable Hannah Fleet.
Jordan is forced to deal with the aftermath of Polly's death on his last shift as joint clinical lead, and Miriam is visited by her step-grandson.
An administrative error throws the ED into turmoil and puts a boy's life in danger, while Mads encounters an aggressive taxi driver.
Tess resorts to drastic measures to avoid losing another patient.
Mads comes face to face with the taxi driver who assaulted her, and nurse Linda Andrews returns to join the overworked ED team.
Linda struggles to gain the team's respect on her first day as clinical-nurse manager, and Dylan is apprehensive about returning to work following Polly's death.
A comment from Jordan causes Adam to worry he has become too detached from his patients, while Jeff agrees to let troubled teen Karl shadow him.
Ruth has an interview to determine whether she will be fully reinstated as a doctor, while Linda clashes with Tess over management styles. S25 Ep42
Adam is tempted to bypass normal hospital procedure to help a known sex offender, and Mads is distraught to learn her new friend is the wife of her attacker Ash. S25 Ep43
Adam becomes involved in the case of two warring brothers, one of whom is dying from asbestos poisoning brought on by working for the family business. S25 Ep44
A couple at a pharmacy are terrorised by a heroin addict desperate to obtain methadone, and Ash steps up his threatening behaviour against Mads. S25 Ep45
A nervous Ruth returns to clinical duties for a probationary period, while Adam's moralistic attitude threatens to affect his medical judgement. S25 Ep46